Bioinformatics: A primer - download pdf or read online

By P. Narayanan

ISBN-10: 8122416101

ISBN-13: 9788122416107

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Shape and rigidity of the particle. Pore size of the separation matrix. ). Optimization of these parameters leads to greater flexibility and versatility of the electrophoresis methods. Any electrophoretic setup consists of at least two components— an electrophoresis unit, and a power pack. The choice of the separating medium depends on the purpose. Paper: Cheap and easy to use, but resolution is poor. Cellulose acetate: Minimal adsorption and faster and clear separation. Better than paper and routinely used in clinical analysis of serum and other body fluid samples.

The polymerization process is bi-directional. The leading strand is polymerized continuously, and the lagging strand discontinuously (Okazaki fragments). As a result, while one RNA primer is required for the leading strand, each Okazaki fragment requires a RNA primer. The RNA portion of the RNA-DNA hybrid is hydrolyzed by DNA pol I (Fig. 15). DNA ligase joins the Okazaki fragments. 16. Features of Nucleic Acids 23 Fig. 11 DNA Synthesis at the Replication Fork Fig. 12 Initiation of DNA Synthesis by an RNA Primer Fig.

2) Since the energy of a charged species is affected by an electric field, the energy of an ion or dipole will be affected by the presence of neighboring ions or dipoles. The interaction energy between permanent dipoles (polar molecules, m π 0) depends on the spatial orientation of the dipoles (Fig. 14). Fig. 3) Average energy of interaction for randomly distributed dipoles is given by Keeson equation. 4) (D = dielectric constant; r = distance between two dipoles; k = Boltzmann constant; T = absolute temperature).

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Bioinformatics: A primer by P. Narayanan

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